RBM15 Knockout cell line (AC16)

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location： Home > Products > KO Cell Lines > RBM15 Knockout cell line (AC16)

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RBM15 Knockout cell line (AC16) Order

RBM15 Knockout cell line (AC16)
Catalog#
YKO-H823
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RBM15 Knockout cell line (AC16)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RBM15 Knockout cell line (AC16)
Morphology
Epithelial-like, adherent
Passage ratio
1：3-1：4
Species
Human
Gene Name
RBM15
Gene ID
64783
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM/F12+10% FBS
Target region
Knockout region: Exon 1 (based on transcript RBM15-201)
Validation: PCR+Sanger Sequencing
RBM15 Knockout cell line (AC16) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:AC16		 STR Info (Cell bank)
Cell Line:AC16 [Human hybrid]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 911911
D13S317 12131213
D7S820 101112.0101112.0
D16S539 11131113
VWA 16181618
TH01 789.3789.3
AMEL XXXX
TPOX 11111111
CSF1PO 91112.091112.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RBM15 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the AC16 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RBM15 gene knockout RBM15 Knockout cell line (AC16) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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